SF3B3 Polyclonal Antibody-BS70723

SF3B3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70723-50, BS70723-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15393

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Background: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SF3B3 polyclonal antibody detects endogenous levels of SF3B3 protein.

Bioworld Molecular Weight: ~ 136 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of mouse tissues, using SF3B3 antibody., Immunohistochemistry of paraffin-embedded rat brain using SF3B3 antibody at dilution of 1:200 (40x lens)., Immunohistochemistry of paraffin-embedded rat kidney using SF3B3 antibody at dilution of 1:200 (40x lens).

Alternative Name: RSE1; SAP130; SF3b130; STAF130;

Immunogen: Recombinant protein of human SF3B3.

Conjugate: Unconjugated

Modification: Unmodified