SF3B3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91230-50, BS91230-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q15393(Human) Q921M3(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500-1:1,000
ICC:1:50-1:100
IHC:1:50-1:200
FC:1:50-1:100
Background: SF3B is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B contains the spliceosomal proteins SAP 49, SAP 130 (also known as SF3B3), SAP 145 and SAP 155. SF3B3, SAP 145 and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SF3B3 and SAP 155 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: SF3B3 polyclonal antibody detects endogenous levels of SF3B3 protein.
Bioworld Molecular Weight: 136 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of SF3B3 on Jurkat and Hela cells lysates using anti-SF3B3 antibody at 1/1,000 dilution., ICC staining SF3B3 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: SF3b130, SF3B3, KIAA0017, SAP130, KIAA0017, Pre mRNA splicing factor SF3b 130 kDa subunit, Pre-mRNA-splicing factor SF3b 130 kDa subunit, RSE1, SAP 130, SAP130, SF3b130, SF3B3, SF3B3, Spliceosome associated protein 130, Spliceosome-associated protein 130, Splicing factor 3b subunit 3 130kD, Splicing factor 3B subunit 3, STAF130,
Immunogen: Recombinant protein within the C-terminus of human SF3B3.
Conjugate: Unconjugated
Modification: Unmodified