SFPQ Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91231-50, BS91231-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P23246(Human) Q8VIJ6(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC ICC IF
All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
ICC:1:10-1:50
Background: DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: SFPQ polyclonal antibody detects endogenous levels of SFPQ protein.
Bioworld Molecular Weight: 100 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of SFPQ on different lysates using anti-SFPQ antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: Daudi
Lane 3: Mouse testis
Lane 4: Mouse lung, Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SFPQ antibody. Counter stained with hematoxylin.
Alternative Name: hPOMp100, PSF, PTB-associated-splicing factor, SFPQ, 100 kDa DNA pairing protein, 100 kDa DNA-pairing protein, 100 kDa subunit, DNA binding p52/p100 complex 100 kDa subunit, DNA-binding p52/p100 complex, hPOMp100, Polypyrimidine tract binding protein associated splicing factor, Polypyrimidine tract-binding protein-associated-splicing factor, POMP100, PPP1R140, proline- and glutamine-rich, Protein phosphatase 1 regulatory subunit 140, PSF, PTB associated splicing factor, PTB-associated-splicing factor, Sfpq, SFPQ, Splicing factor, Splicing factor proline and glutamine rich, Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated), Splicing factor proline/glutamine rich,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified