SFPQ Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-62211-60, E-AB-62211-120, E-AB-62211-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: SFPQ
Target Synonym: SFPQ; POMP100; PPP1R140; PSF
Research Areas: Cancer, Epigenetics and Nuclear Signaling
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC, IF
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P23246
Background: DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human SFPQ (NP_005057.1).
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100
Calculated MW: 72kDa/76kDa
Observed MW: 115kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only