Product Description

SFRP2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7759-50, BS7759-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96HF1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC/IF: 1:50~1:200

Background: The frizzled gene, originally identified in Drosophila melanogaster, was shown to be involved in the development of tissue polarity. The mammalian homolog of frizzled, as well as several secreted, mammalian, frizzled-related proteins such as FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified. The frizzled proteins contain seven transmembrane domains and a cysteine-rich domain in the extra carboxy-terminal Ser/Thr-xxx-Val motif, and they function as receptors for Wnt. The frizzled-1 gene maps to human chromosome 7q21 and is expressed in adult heart, placenta, lung, kidney, pancreas, prostate and ovary, as well as in fetal lung and kidney. Frizzled-2 is expressed in adult heart and fetal brain, lung and kidney. The frizzled-related proteins FRP-1, FRP-2, FRP-3, FRP-4 and SARP3 are secreted proteins that contain regions of homology to the cysteine-rich, ligand-binding domain of frizzled and a conserved, hydrophilic carboxy-terminus. The gene encoding human SARP3 maps to chromosome 4q31.3 and is expressed in retinal pigment epithelium (RPE) and pancreas, while expression of FRP-1, 2 and 4 is high in developing tissues. The FRPs/SARPs are involved in the Wnt signaling pathway by regulating the intracellular levels of β-catenin.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SFRP2 polyclonal antibody detects endogenous levels of SFRP2 protein.

Bioworld Molecular Weight: ~ 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cells, using SFRP2 antibody., Immunohistochemistry of paraffin-embedded rat brain using SFRP2 antibody at dilution of 1:200 (400x lens)., Immunohistochemistry of paraffin-embedded mouse heart using SFRP2 antibody at dilution of 1:200 (400x lens).

Alternative Name: Frizzled related protein 2;
FRP 2;
FRP-2;
FRP2;
MGC128911;
MGC153618;
MGC53344;
SARP 1;
SARP-1;
SARP1;
SDF 5;
SDF5;
Secreted apoptosis related protein 1;
Secreted apoptosis-related protein 1;
Secreted frizzled related protein 2;
Secreted frizzled-related protein 2;
sFRP 2;
sFRP-2;
SFRP2;
Sfrp2 protein;

Immunogen: Recombinant full length Human SFRP2.

Conjugate: Unconjugated

Modification: Unmodified