Product Description

SFRS15 (V641) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3953-50, BS3953-100

Sizes: 50µl, 100µl

Swiss-Prot: O95104

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The family of SR factors all contain one or more RNA recognition motifs (RRM) and an SR-rich domain. They are not only essential for constitutive splicing, but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. Splicing factor arginine/serine-rich 15 (SFRS15), also designated CTD-binding SR-like protein RA4, contains one RRM and one SR-rich domains. SFRS15 interacts with C-terminal repetitive domain (CTD) of Pol II and is believed to functionally and physically link transcription and pre-mRNA processing. Localized to the nucleus, SFRS15 is expressed as two isoforms produced by alternative splicing.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SFRS15 (V641) polyclonal antibody detects endogenous levels of SFRS15 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SFRS15 (V641) pAb at 1:1000 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: CT26 whole cell lysate (40ug)
Lane 3: HCT116 whole cell lysate (40ug)
Lane 4: SGC7901 whole cell lysate (40ug)
Lane 5: HEK293T whole cell lysate (20ug), Immunohistochemistry (IHC) analyzes of SFRS15 (V641) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 126 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Splicing factor, arginine/serine-rich 15; CTD-binding SR-like protein RA4; SR-related and CTD-associated factor 4; SCAF4; KIAA1172; SFRS15

Immunogen: Synthetic peptide, corresponding to amino acids 611-660 of Human SFRS15.

Conjugate: Unconjugated

Modification: Unmodified