Product Description

SFRS3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91235-50, BS91235-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P84103 Human;P84104 Mouse;Entrez Gene: 361814 Rat

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:100-1:500

Background: Splicing factor that specifically promotes exon-inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. Also functions as export adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244). RNA-binding is semi-sequence specific.

Purification and Purity: Protein A purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: SFRS3 polyclonal antibody detects endogenous levels of SFRS3 protein.

Bioworld Molecular Weight: 19 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SFRS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse testis tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: A431 cell lysate
Lane 4: SH-SY-5Y cell lysate
Lane 5: SiHa cell lysate
Lane 6: 293 cell lysate
Lane 7: Hela cell lysate
Lane 8: HepG2 cell lysate
Lane 9: A549 cell lysate, Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SFRS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-47 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Alternative Name: SRSF3, SFRS3, SRP20, arginine/serine-rich 3, Pre mRNA splicing factor SRP20, Pre-mRNA-splicing factor SRP20, Serine/arginine-rich splicing factor 3, Splicing factor, Splicing factor arginine/serine rich 20 kD, Splicing factor arginine/serine rich 3, SRp20, SRSF3, SRSF3, X16 antibody

Immunogen: Full length recombinant protein.

Conjugate: Unconjugated

Modification: Unmodified