Sgo2 rabbit pAb - ES4904

Sgo2 rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES4904-50, ES4904-100

Citations, Manuals and MSDS Available upon request.

Background: function:During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis (By similarity). May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., sequence caution:Contaminating sequence. Potential poly-A sequence., similarity:Belongs to the shugoshin family., subcellular location:In Hela cells, localizes at centromeres throughout prophase until metaphase and disappears at anaphase., subunit:Directly interacts with PPP2CA.,

Alternate Name: SGOL2; Shugoshin-like 2; Shugoshin-2; Sgo2; Tripin

Source: Rabbit

Applications: IHC; IF; ELISA

Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Reactivity: Human; Rat; Mouse;

Immunogen: The antiserum was produced against synthesized peptide derived from human SGOL2. AA range:681-730

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Human Gene ID: 151246

Human SWISS Prot NO: Q562F6

Subcellular Location: Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. During meiosis I, accumulates at centromeres during diplotene, and colocalizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres (By similarity). SGO2 and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost (By similarity). During meiosis II, it shows a striking tension-dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis (By similarity). In Hela cells, localizes at centromeres throughout prophase until metaphase and disappears at anaphase (PubMed:17485487). Centromeric localization requires the presence of BUB1 and AUKRB (PubMed:17485487).

Research Use Only