Product Description

SH-PTP2 (phospho Tyr542) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1455-50, ES1455-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: PTPN11; PTP2C; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human SHP-2 around the phosphorylation site of Tyr542. AA range:508-557

Isotype: IgG

Target: SH-PTP2,

Specificity: Phospho-SH-PTP2 (Y542) Polyclonal Antibody detects endogenous levels of SH-PTP2 protein only when phosphorylated at Y542.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed, with highest levels in heart, brain, and skeletal muscle.

Observed Band (KD): 70kD

Fields: Ras signaling pathway, Phospholipase D signaling pathway, Axon guidance, C-type lectin receptor signaling pathway, JAK-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Leukocyte transendothelial migration, Neurotrophin signaling pathway, Adipocytokine signaling pathway, Insulin resistance, Epithelial cell signaling in Helicobacter pylori infection, Pathogenic Escherichia coli infection, Herpes simplex virus 1 infection, Proteoglycans in cancer, Chemical carcinogenesis - reactive oxygen species, Renal cell carcinoma, Chronic myeloid leukemia, PD-L1 expression and PD-1 checkpoint pathway in cancer

Function: Catalytic Activity: Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. Disease: Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor. Disease: Defects in PTPN11 are a cause of Noonan-like syndrome [MIM:163955]; also known as Noonan-like/multiple giant cell lesion syndrome. It is an autosomal dominant disorder characterized by Noonan features associates with giant cell lesions of bone and soft tissue. Disease: Defects in PTPN11 are the cause of LEOPARD syndrome [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan

Subcellular Location: Cytoplasm Nucleus

Storage and Stability: -20°C/1 year

Gene Name: PTPN11

Protein Name: Tyrosine-protein phosphatase non-receptor type 11

Human Gene ID: 5781

Human SwissProt Number: Q06124

Mouse Gene ID: 19247

Mouse SwissProt Number: P35235

Rat Gene ID: 25622

Rat SwissProt Number: P41499

Research Use Only