Product Description

SH3BGRL Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS77313-50, BS77313-100

Swiss-Prot: O75368

Host: Rabbit

SH3BGRL Polyclonal Antibody-BS77313

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:100 - 1:200 | IF/ICC,1:50 - 1:200

Background: Predicted to enable SH3 domain binding activity. Located in extracellular exosome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 16kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SH3BGRL antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of U-2 OS cells using SH3BGRL antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: HEL-S-115; SH3BGR; SH3BGRL

Immunogen: Recombinant fusion protein of human SH3BGRL(NP_003013.1).

Modification: Unmodified

Conjugate: Unconjugated