SHARPIN Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-90300-60, E-AB-90300-120, E-AB-90300-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: SHARPIN
Target Synonym: SHARPIN; SIPL1; sharpin
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Rat
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q9H0F6
Background: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human SHARPIN
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000
Calculated MW: 33kDa/39kDa
Observed MW: 40kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only