Shc (phospho-Y349) Polyclonal Antibody-BS4250

Shc (phospho-Y349) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4250-50, BS4250-100

Swiss-Prot: P29353

Host: Rabbit

Shc (phospho-Y349) Polyclonal Antibody-BS4250

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Shc (Y349) polyclonal antibody detects endogenous levels of Shc protein only when phosphorylated at Tyr349.

Bioworld Molecular Weight: ~ 46, 52, 66 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-Shc (Y349) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: HEK293T treated with EGF (100ng/ml,15 minutes) whole cell lysate
Lane 3: HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate
Lane 4: The Kidney tissue lysate of Mouse
Lane 5: The Kidney tissue lysate of Rat, Immunohistochemistry (IHC) analyzes of p-Shc (Y349) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Alternative Name: SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1; SHC1; SHC; SHCA

Immunogen: Synthetic phosphopeptide derived from human Shc around the phosphorylation site of Tyrosine 349.

Modification: Phosphorylation

Conjugate: Unconjugated