SHH Polyclonal Antibody-BS91237

SHH Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91237-50, BS91237-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15465(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:200-1:500
FC:1:50-1:100

Background: The Drosophila segment polarity gene hedgehog (hh) encodes a precursor protein which undergoes autocleavage to generate amino- and carboxy-terminal peptides. Both proteins are secreted and appear to function in embryonic and imaginal disc patterning. Several vertebrate homologs of Drosophila hh have been identified. These include Sonic hedgehog (Shh) (alternatively designated Vhh-1), Desert hedgehog (Dhh) and Indian hedgehog (Ihh). Each contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Shh resembles Drosophila hh in that it is processed to generate an amino-terminal secreted peptide that is retained at or near the cell surface and a carboxy-terminal glycosylated more diffusible peptide.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SHH polyclonal antibody detects endogenous levels of SHH protein.

Bioworld Molecular Weight: 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2, ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SHH, HHG 1, HHG-1, HHG1, HLP 3, HLP3, Holoprosencephaly 3, HPE 3, HPE3, MCOPCB5, shh, SHH, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog homolog, Sonic hedgehog protein, Sonic hedgehog protein C-product, TPT, TPTPS,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified