Shh Polyclonal Antibody - BT-AP07978

Shh Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP07978-20, BT-AP07978-50, BT-AP07978-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube| the anterior-posterior limb axis| and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila| this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly| the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product| restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE)| a d

Research Areas: Developmental biology; Cancer; Epigenetics and nuclear signaling; Metabolism; Stem cells

Target Protein: SHH

Applications: IHC-p, IF, WB

Reactivity: Human, Mouse, Rat

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: Shh

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

UniProt Accession: Human: Q15465

Synonyms: Shh; Sonic hedgehog protein; SHH; HHG-1; Sonic hedgehog protein N-product; Sonic hedgehog protein C-product;

GeneID: Human: 6469

Research Use Only