Product Description

Shh Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA29801-50, RA29801-100

Citations, Manuals and MSDS Available upon request.

Background: sonic hedgehog (SHH) Homo sapiens This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a d

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage Instructions: -20°C/1 year

Recommended dilutions: IHC-p 1:50-200, WB 1:500-2000

Alternative Names: Sonic hedgehog protein (SHH;HHG-1) [Cleaved into: Sonic hedgehog protein N-product; Sonic hedgehog protein C-product]

Applications: IHC-p; IF (paraffin section) ; WB

Species Cross-Reactivity: Human; Mouse; Rat

GeneID (Human): 6469

Protein MW (KDa): 40

SWISS: Q15465

Source: Rabbit

Research Use Only