Shh rabbit pAb - ES13122

Shh rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES13122-50, ES13122-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a d

Alternate Name: Sonic hedgehog protein (SHH; HHG-1) [Cleaved into: Sonic hedgehog protein N-product; Sonic hedgehog protein C-product]

Source: Rabbit

Applications: IHC; IF; WB

Dilution: IHC-p 1:50-200, WB 1:500-2000

Reactivity: Human; Mouse; Rat

Immunogen: Synthesized peptide derived from human Shh

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 40kD

Human Gene ID: 6469

Human SWISS Prot NO: Q15465

Subcellular Location: Endoplasmic reticulum membrane. Golgi apparatus membrane. Co-localizes with HHAT in the ER and Golgi membrane.; [Sonic hedgehog protein N-product]: Cell membrane; Lipid-anchor. The dual-lipidated sonic hedgehog protein N-product (ShhNp) is firmly tethered to the cell membrane where it forms multimers (PubMed:24522195). Further solubilization and release from the cell surface seem to be achieved through different mechanisms, including the interaction with DISP1 and SCUBE2, movement by lipoprotein particles, transport by cellular extensions called cytonemes or by the proteolytic removal of both terminal lipidated peptides (PubMed:26875496, PubMed:24522195).

Research Use Only