Product Description

SHMT2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80361-50, BS80361-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P34897

Host: Rabbit

Reactivity: Human

Applications: IHC, IF/ICC, IP,

All Applications: IHC,1:50 - 1:100 | IF/ICC1:50 - 1:200 | IP,1:50 - 1:200

Background: This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded rat spleen using SHMT2 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse spleen using SHMT2 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of L929 cells using SHMT2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: SHMT2; GLYA; HEL-S-51e; SHMT; serine hydroxymethyltransferase 2

Immunogen: Recombinant fusion protein of human SHMT2(NP_005403.2).

Conjugate: Unconjugated

Modification: Unmodified