Product Description

Shugoshin 1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67202-50, BS67202-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubμle to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regμlating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: SGO1, Shugoshin-like 1, hSgo1, Serologically defined breast cancer antigen NY-BR-85

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Shugoshin 1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 163% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Shugoshin 1 protein.

Molecular Weight: ~ 64 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q5FBB7

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only