Product Description

Siah-2 (E266) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2425-50, BS2425-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regμlates cell responses to stress and cytokines through the regμlation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1α prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-α-dependent induction of JNK activity and transcriptional activation of NFκB. SIAH-2 nμll mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: E3 ubiquitin-protein ligase SIAH2, Seven in absentia homolog 2, Siah-2, hSiah2, SIAH2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 241-290 of Human Siah-2.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Siah-2 (E266) polyclonal antibody detects endogenous levels of Siah-2 protein.

Molecular Weight: ~ 38 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O43255

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only