Product Description

SIRT2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91245-50, BS91245-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8IXJ6(Human) Q8VDQ8(Mouse) Q5RJQ4(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF, IP, FC

All Applications: ICC:1:50-1:200
FC:1:50-1:100

Background: The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in a NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT proteins may function via mono-ADP-ribosylation of proteins. SIRT2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SIRT2 polyclonal antibody detects endogenous levels of SIRT2 protein.

Bioworld Molecular Weight: 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining SIRT2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining SIRT2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SIRT2, FLJ35621, FLJ37491, NAD dependent deacetylase sirtuin 2, NAD-dependent deacetylase sirtuin-2, NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, Silencing information regulator 2 like, Silent information regulator 2, SIR2, SIR2 like protein 2, Sir2 related protein type 2, SIR2, S. cerevisiae, homolog-loke 2, SIR2-like protein 2, SIR2L, SIR2L2, SIRT2, SIRT2, Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae), Sirtuin 2, Sirtuin type 2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified