Product Description

SIRT3 Polyclonal Antibody

Catalogue Numbers: BS62525-50, BS62525-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NTG7

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB:1:500~1:1000

Background: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain. The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product. This processing is most-likely necessary for its enzymatic activity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SIRT3 polyclonal antibody detects endogenous levels of SIRT3 protein.

Extra Notes: Western blot (WB) analysis of SIRT3 polyclonal antibody at 1:500 dilution
LaneA:HCT116 whole cell lysate

Bioworld Molecular Weight: ~ 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial; hSIRT3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3; SIRT3; SIR2L3

Immunogen: Synthetic peptide, corresponding to Human SIRT3.

Conjugate: Unconjugated

Modification: Unmodified