Product Description

SIX1 Polyclonal Antibody

Catalogue Numbers: BS62393-50, BS62393-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: Q15475

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB:1:500~1:1000

Background: The Six proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six2, Six4 (AREC3) and Six5 bind to the same DNA sequence, indicating that they may regulate the same target genes. Six1 and Sx4 are both capable of transactivating MEF3 site containing reporter genes, such as myogenin. It has been demonstrated that alterations to homeobox-containing genes may result in cancer. Six1 expression has been shown to be absent or low in normal adult tissues, although it is expressed in several tumor types, including breast carcinoma. Six1 overexpression has been shown to abrogate the G2 cell cycle checkpoint.

Purification and Purity: The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SIX1 polyclonal antibody detects endogenous levels of SIX1 protein.

Extra Notes: Western blot (WB) analysis of SIX1 polyclonal antibody at 1:500 dilution
LaneA:PC12 whole cell lysate
LaneB:MEF whole cell lysate
LaneC:COS-7 whole cell lysate

Bioworld Molecular Weight: ~ 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Homeobox protein SIX1; Sine oculis homeobox homolog 1; SIX1

Immunogen: Synthetic peptide, corresponding to Human SIX1.

Conjugate: Unconjugated

Modification: Unmodified