Product Description

Six3/6 (D228) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1903-50, BS1903-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The SIX proteins (sine ocμlis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Two of these family members Six3 and Six6 (also designated Optx2 and Six9) are required for the specification and proliferation of the eye field in vertebrates, and, therefore, are the vertebrate homologues most closely related to the Drosophila sine ocμlis protein, which has an essential role in controlling compound eye development. Six3 and Six6 expression largely overlap during development of specific tissues, such as retina, hypothalamus, and pituitary. Deletion of Six3 may be associated with HPE2 disorder, a common, severe malformation of the brain that resμlts from incomplete cleavage of the forebrain during early embryogenesis.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF

Alternate Names: Homeobox protein SIX3, Sine ocμlis homeobox homolog 3, SIX3, HPE2, Homeobox protein SIX6, Homeodomain protein OPTX2, Optic homeobox 2, SIX6, OPTX2, SIX9

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human Six3.

Dilution: WB: 1:500~1:1000
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Six3/6 (D228) polyclonal antibody detects endogenous levels of Six3 protein (35 KDa), and also detects Six6 protein (28 KDa).

Molecular Weight: ~ 28 kDa (Six6), 35 kDa (Six3)

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O95343/O95475

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only