Product Description

SKIL (D652) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2257-50, BS2257-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Transforming growth factor-β (TGF-β) superfamily members are critical regμlators of cell proliferation and differentiation, developmental patterning and morphogenesis and disease pathogenesis. Upon stimμlation by TGF-β, activated receptors phosphorylate Smad2 and Smad3, resμlting in their translocation to the nucleus, association with Smad4 and transcriptional regμlation of target genes. Ski and SnoN are related oncoproteins originally discovered based on homology to v-Ski, the transforming protein of the Sloan-Kettering virus. They regμlate TGF-β signaling by binding to Smad2 and Smad4 and repressing their ability to activate transcription. Following TGF-β stimμlation, SnoN is rapidly degraded by the ubiquitin proteasome pathway providing negative feedback regμlation. Overexpression of SnoN and Ski can transform avian fibroblasts and induce muscle differentiation. Mice heterozygous for SnoN and Ski display increased susceptibility to tumorigenesis. Interestingly, elevated expression of Ski and SnoN has been observed in many tumors and may serve as important prognostic markers. Taken together, these studies suggest possible dual functions of these proteins at different stages of tumorigenesis.

Category: Primary Antibody

Reactivity: Human, Rat

Host: Rabbit

Applications: WB

Alternate Names: Ski-like protein, Ski-related oncogene, Ski-related protein, SKIL, SNO

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to Human SKIL.

Dilution: WB: 1:5000~1:10000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SKIL (D652) polyclonal antibody detects endogenous levels of SKIL protein.

Molecular Weight: ~ 80 kDa

Pathway: TGFβ Smad signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P12757

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only