Skp1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91249-50, BS91249-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P63208(Human) Q9WTX5(Mouse) Q6PEC4(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF FC
All Applications: WB:1:500
ICC:1:50-1:100
FC:1:50-1:100
Background: Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Skp1 polyclonal antibody detects endogenous levels of Skp1 protein.
Bioworld Molecular Weight: 19 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Skp1 on PC-12 cell using anti-Skp1 antibody at 1/1,000 dilution., ICC staining Skp1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: p19A, OCP-2, OCP-II, SKP1, EMC19, OCP2, SKP1A, TCEB1L, Cyclin A/CDK2 associated p19, Cyclin A/CDK2 associated protein p19, Cyclin-A/CDK2-associated protein p19, EMC19, MGC34403, OCP 2, OCP II, OCP II protein, OCP-2, OCP-II, OCP2, OCPII, Organ of Corti protein 2, Organ of Corti protein II, OTTHUMP00000159379, OTTHUMP00000159380, OTTHUMP00000228275, OTTHUMP00000228276, OTTHUMP00000228278, OTTHUMP00000228279, OTTHUMP00000228281, p19A, p19skp1, RNA polymerase II elongation factor like protein, RNA polymerase II elongation factor like protein OCP2, RNA polymerase II elongation factor-like protein, S phase kinase associated protein 1, S phase kinase associated protein 1A, S phase kinase associated protein 1A p19A, S-phase kinase-associated protein 1, SIII, Skp 1, SKP 1A, skp1, SKP1, SKP1A, TCEB1L, Transcription Elongation Factor B 1 like, Transcription elongation factor B, Transcription elongation factor B SIII polypeptide 1 like,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified