Skp2 p45 (P399) Polyclonal Antibody-BS1325

Skp2 p45 (P399) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1325-50, BS1325-100

Sizes: 50µl, 100µl

Swiss-Prot: Q13309

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF

Background: The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase 2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F, and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SKP2 p45 (P399) polyclonal antibody detects endogenous levels of SKP2 p45 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SKP2 at 1:500 dilution
Lane 1: EC9706 whole cell lysate (40ug)
Lane 2: HepG2 whole cell lysate (40ug)
Lane 3: U-87MG whole cell lysate (40ug)
Lane 4: A375 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Skp2 p45 (P399) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 55 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: S-phase kinase-associated protein 2; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2; SKP2; FBXL1

Immunogen: Synthetic peptide, corresponding to amino acids 369-419 of Human Skp2 p45.

Conjugate: Unconjugated

Modification: Unmodified