SLC12A3 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS77693-50, BS77693-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: P55017 Host: Rabbit Reactivity: Human, Mouse, Monkey Applications: WB All Applications: WB,1:500 - 1:2000 Background: This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Extra Notes: Western blot analysis of extracts of various cell lines, using SLC12A3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. Note: For research use only, not for use in diagnostic procedure. Bioworld Molecular Weight: 100-170KDa Alternative Name: NCC; NCCT; TSC; SLC12A3 Immunogen: Recombinant fusion protein of human SLC12A3(NP_000330.2). Conjugate: Unconjugated Modification: Unmodified