Product Description

SLC8A1 Polyclonal Antibody

Catalogue Numbers: BS5816-50, BS5816-100

Sizes: 50µl, 100µl

Alternative Name: Sodium/calcium exchanger 1; Na(+)/Ca(2+)-exchange protein 1; Solute carrier family 8 member 1; CNC; NCX1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P32418

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Sodium/calcium exchanger proteins are integral membrane proteins primarily seen in cardiac cells. In cardiac myocytes, the concentration of Ca2+ alternates between low levels during relaxation and high levels during contraction. The Na+/Ca2+ exchanger 1 (NCX1) protein mediates Ca2+ extrusion from cardiac cells during relaxation. Four NCX1 isoforms (NCX1.1, NCX1.3, NCX1.7, and NCX1.10) result from alternate splicing. NCX1 mRNA is present at high levels in the heart, with lower levels present in the brain. NCX2 is most abundantly expressed in brain, in contrast the the broader distribution of NCX1, which is also expressed in heart, kidney, lung, smooth and skeletal muscle. The difference in expression for the transporter subtypes is believed to reflect differences in their functional roles. Regulation mechanisms for these exchanger proteins have not been fully characterized.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SLC8A1 polyclonal antibody detects endogenous levels of SLC8A1 protein.

Bioworld Molecular Weight: ~ 108 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SLC8A1 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 336-381 of Human SLC8A1.

Conjugate: Unconjugated

Modification: Unmodified