Product Description

Smad2/3 (6F7) Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB3250-50, MB3250-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Members of the Smad family of signal transduction molecμles are components of a critical intracellμlar pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regμlated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 . Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regμlate transcriptional responses .

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Mouse

Applications: IHC-P, WB

Alternate Names: SMAD3, MADH3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Recombinant Protein of Smad3

Dilution: WB: 1/500-1/1000 IHC: 1/50-1/100

Purification: Affinity Purified

Specificity: IgG

Molecular Weight: Calcμlated MW: 52 kDa, Observed MW: 52, 60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P84022/Q15796

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only