Smad2/3 (6F7) Monoclonal Antibody-MB3250

Smad2/3 (6F7) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3250-50, MB3250-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P84022/Q15796

Host: Mouse

Reactivity: Human, Rat, Mouse

Applications: IHC-P, WB,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 . Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses .

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 52 kDa; Observed MW: 52,60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry analysis of paraffin-embedded Human Live rTissue using Smad2/3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemical analysis of paraffin-embedded Human tonsils using Smad2/3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Western blot analysis of Smad2/3 in C6, MCF-7, Jurkat and 3T3 lysates using Smad2/3 antibody

Alternative Name: SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Immunogen: Recombinant Protein of Smad3

Conjugate: Unconjugated

Modification: Unmodified