Product Description

SMAD2 (Phospho-S255) Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66303-50, MB66303-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Members of the Smad family of signal transduction molecμles are components of a critical intracellμlar pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regμlated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regμlate transcriptional responses.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC, IP

Alternate Names: MADH2, MADR2, Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser255 of human Smad2

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of SMAD2 (pS255) protein.

Molecular Weight: ~ 55 kDa

Pathway: Tumor Angiogenesis, ESC Pluripotency and Differentiation Signaling Pathway, TGFβ Smad signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q15796

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only