Product Description

Smad3 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10356-50, MB10356-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P84022

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 48 kDa; Observed MW: 52 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of Smad3 in A549 using Smad3 antibody, and DAPI., Western blot analysis of Smad3 in Jurkat, C6, Hela lysates using Smad3 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Smad3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Immunogen: A synthetic peptide of human Smad3

Conjugate: Unconjugated

Modification: Unmodified