Smad3 Polyclonal Antibody-BS80100

Smad3 Polyclonal Antibody Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Sizes: 50µl, 100µl Catalogue Numbers: BS80100-50, BS80100-100 Swiss-Prot: P84022 Host: Rabbit Smad3 Polyclonal Antibody-BS80100 Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Reactivity: Human, Mouse, Rat Applications: WB, IF/ICC All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ' mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Specificity: Unmodification Bioworld Molecular Weight: 52KDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s., Immunofluorescence analysis of NIH/3T3 cells using Smad3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining. Alternative Name: HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; SMAD3 Immunogen: Recombinant fusion protein of human Smad3(NP_005893.1). Modification: Unmodified Conjugate: Unconjugated