Product Description

Smad7 Polyclonal Antibody

Catalogue Numbers: BS60366-50, BS60366-100

Sizes: 50µl, 100µl

Alternative Name: Mothers against decapentaplegic homolog 7; MAD homolog 7; Mothers against DPP homolog 7; Mothers against decapentaplegic homolog 8; MAD homolog 8; Mothers against DPP homolog 8; SMAD family member 7; SMAD 7; Smad7; hSMAD7; MADH7; MADH8

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15105

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFß and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFß signaling by interfering with TGFß-mediated phosphorylation of other Smad family members.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Smad7 polyclonal antibody detects endogenous levels of Smad7 protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Smad7 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human Smad7.

Conjugate: Unconjugated

Modification: Unmodified