SMAD7 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS67756-50, BS67756-100 Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Swiss-Prot: O15105 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB, IHC, IF/ICC All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200) Background: Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been implicated as downstream effectors of TGFb/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFb and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFb signaling by interfering with TGFb-mediated phosphorylation of other Smad family members. Purification and Purity: The antibody was purified by immunogen affinity chromatography. Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Recognizes endogenous levels of SMAD7 protein. Bioworld Molecular Weight: ~ 47 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of SMAD7 expression in mouse brain (A) whole cell lysates., Immunohistochemical analysis of SMAD7 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of SMAD7 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. Alternative Name: MADH7; MADH8; Mothers against decapentaplegic homolog 7; MAD homolog 7; Mothers against DPP homolog 7; Mothers against decapentaplegic homolog 8; MAD homolog 8; Mothers against DPP homolog 8; SMAD family member 7; SMAD 7; Smad7; hSMAD7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SMAD7. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified