Product Description

SMC3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91259-50, BS91259-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UQE7(Human) Q9CW03(Mouse) P97690(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein and Bamacan for the secreted proteoglycan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan expressed in testis and brain. The secreted proteoglycan contains several glycosylation sites and is thought to play a role in basement membrane physiology.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SMC3 polyclonal antibody detects endogenous levels of SMC3 protein.

Bioworld Molecular Weight: 141 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution.
Positive control:
Line1: HepG2
Line2: NIH-3T3
Line3:PC12, ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SMC protein 3, SMC-3, Bamacan, hCAP, BAM, Bamacan, Basement membrane associated chondroitin proteoglycan, Basement membrane-associated chondroitin proteoglycan, BMH, CDLS3, chondroitin sulfate proteoglycan 6 (bamacan), Chondroitin sulfate proteoglycan 6, Chromosome associated polypeptide, Chromosome-associated polypeptide, CSPG 6, CSPG6, hCAP, Human chromosome associated polypeptide, im:7142991, SMC 3, SMC protein 3, SMC-3, smc3, SMC3, SMC3L1, Structural maintenance of chromosome 3, Structural maintenance of chromosomes 3, Structural maintenance of chromosomes protein 3, u:fb22e01, wu:fc30d07,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified