SMG7 (K548) Polyclonal Antibody-BS2112

SMG7 (K548) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2112-50, BS2112-100

Sizes: 50µl, 100µl

Swiss-Prot: Q92540

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: In humans, NMD depends on RNA-dependent ATPase and 5' to 3' helicase UPF1, plus six other proteins designated SMG1, SMG5, SMG6, SMG7, UPF2 and UPF3. SMG5, SMG7 and UPF1 localize to cytoplasmic foci called P-bodies, while SMG5, SMG6 and SMG7 target UPF1 for dephosphorylation. SMG7 may also act as an adaptor in targeting mRNAs associated with phosphorylated UPF1 for degradation. SMG7 provides a link between the NMD pathway and mRNA degradation machinery by forming a complex with the proteins SMG5 and UPF1, interacting with them via its N-terminal domain, and targeting bound reporter transcripts for decay via its C-terminal domain. SMG7 contains a 14-3-3-like domain, and residues that bind phosphoserine-containing peptides in 14-3-3 proteins are conserved at the equivalent positions in SMG7.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SMG7 (K548) polyclonal antibody detects endogenous levels of SMG7 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SMG7 (K548) pAb at 1:500 dilution
Lane 1: K562 whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: U-87MG whole cell lysate (40ug)
Lane 4: SGC7901 whole cell lysate (40ug)
Lane 5: PC12 whole cell lysate (40ug)
Lane 6: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of SMG7 (K548) pAb in paraffin-embedded human tonsil cancer tissue at 1:50.

Bioworld Molecular Weight: ~ 127 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: C1orf16; EST1 like protein C; EST1 telomerase component homolog C; EST1C; ever shorter telomeres 1C; FLJ23717; SGA56M; SMG 7;

Immunogen: Synthetic peptide, corresponding to amino acids 512-564 of Human SMG7.

Conjugate: Unconjugated

Modification: Unmodified