SMIF (S133) Polyclonal Antibody-BS2145

SMIF (S133) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2145-50, BS2145-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9NPI6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Signal transduction of TGF-β superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-β-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-β and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-β-induced nuclear translocation of SMIF (1). The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SMIF (S133) polyclonal antibody detects endogenous levels of SMIF protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SMIF (S133) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: HepG2 whole cell lysate (40ug)
Lane 3: H1792 whole cell lysate (40ug)
Lane 4: AML-12 whole cell lysate (40ug)
Lane 5: PC12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of SMIF (S133) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 75 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: mRNA-decapping enzyme 1A; Smad4-interacting transcriptional co-activator; Transcription factor SMIF; DCP1A; SMIF

Immunogen: Synthetic peptide, corresponding to amino acids 102-150 of Human SMIF.

Conjugate: Unconjugated

Modification: Unmodified