Product Description

Smooth Muscle Actin Polyclonal Antibody

Catalogue Numbers: BS62610-50, BS62610-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: P62736

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC:1:50~1:100

Background: Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Smooth Muscle Actin polyclonal antibody detects endogenous levels of Smooth Muscle Actin protein.

Extra Notes: Western blot (WB) analysis of Smooth Muscle Actin polyclonal antibody at 1:500 dilution
LaneA:HEK293T whole cell lysate(40ug)
LaneB:Hela whole cell lysate(40ug)
LaneC:A2780 whole cell lysate(40ug)
LaneD:The mouse spleen lysate of Mouse(40ug)

Bioworld Molecular Weight: ~ 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein; ACTA2; ACTSA; ACt VS; GIG46

Immunogen: Synthetic peptide, corresponding to Human Smooth Muscle Actin.

Conjugate: Unconjugated

Modification: Unmodified