SMUG1 Polyclonal Antibody-BS5753

SMUG1 Polyclonal Antibody

Catalogue Numbers: BS5753-50, BS5753-100

Sizes: 50µl, 100µl

Alternative Name: Single-strand selective monofunctional uracil DNA glycosylase

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q53HV7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHCICC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
ICC: 1:50~1:200

Background: The base excision repair (BER) pathway removes incorrect bases (uracil) or damaged bases (3-methyladenine) from chromatin. Each BER enzyme system addresses a specific type of base damage. Uracil-DNA glycosylases, UNG2 and SMUG1 (single-strand selective monofunctional uracil DNA glycosylase) remove uracil from both double- and single-stranded DNA in nucleosomes (chromatin core particle). The uracil-excising enzyme family shares structural and functional conservation with minimal sequence conservation. The human SMUG1 gene maps to chromosome 12q13.13.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SMUG1 polyclonal antibody detects endogenous levels of SMUG1 protein.

Bioworld Molecular Weight: ~ 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SMUG1 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 137-182 of Human SMUG1.

Conjugate: Unconjugated

Modification: Unmodified