SMURF2 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-90220-60, E-AB-90220-120, E-AB-90220-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: SMURF2
Target Synonym: SMURF2
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Stem Cells
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q9HAU4
Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby downregulating TGF-beta signaling. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1. Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation. SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation. Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation.
Concentration: 1mg/mL
Immunogen: Recombinant fusion protein of human SMURF2
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:1000-1:2000
Calculated MW: 86kDa
Observed MW: 86kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only