SOD2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91269-50, BS91269-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P04179(Human) P09671(Mouse) P07895(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200
Background: The superoxide dismutase family is composed of three metalloenzymes (SOD-1, SOD-2 and SOD-3) that catalyze the oxido-reduction of reactive oxygen species (ROS) such as superoxide anion. The SOD-2 precursor is a 222 amino acid protein that is encoded by nuclear chromatin, synthesized in the cytosol and imported posttranslationally into the mitochondrial matrix. Unlike SOD-1, which is a homodimeric cytosolic Cu-Zn enzyme, SOD-2 is a homotetrameric manganese enzyme (also known as MnSOD) that functions in the mitochondrion. ROS are implicated in a wide range of degenerative processes, including Alzheimer’s disease, Parkinson’s disease and ischemic heart disease. Homozygous mutant mice, which lack SOD-2, exhibit dilated cardiomyopathy, accumulation of lipid in liver and skeletal muscle, metabolic acidosis, oxidative DNA damage and respiratory chain deficiencies in heart and skeletal muscle. Polymorphisms in the SOD-2 gene have also been implicated in nonfamilial, idiopathic, dilated cardiomyopathy in humans.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: SOD2 polyclonal antibody detects endogenous levels of SOD2 protein.
Bioworld Molecular Weight: 21 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of SOD2 on different lysates using anti-SOD2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse brain
Lane 2: SH-SY-5Y
Lane 3: Human heart, Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SOD2 antibody. Counter stained with hematoxylin.
Alternative Name: SOD2, Indophenoloxidase B, IPO B, IPOB, Manganese containing superoxide dismutase, Manganese SOD, Manganese superoxide dismutase, Mangano superoxide dismutase, Mn SOD, Mn superoxide dismutase, MNSOD, MVCD6, SOD 2, SOD2, SODM, Superoxide dismutase [Mn] mitochondrial, Superoxide dismutase [Mn], mitochondrial, Superoxide dismutase 2 mitochondrial,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified