Product Description

Sonic Hedgehog Recombinant Rabbit mAb

Sizes: 50μl, 100μl

Catalogue Numbers: BS45732-50, BS45732-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistμla with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can resμlt in preaxial polydactyly. [provided by RefSeq, Jμl 2008]

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC, ICC/IF, FC

Alternate Names: TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide of human Sonic Hedgehog

Dilution: WB: 1:1000-1:5000 IHC: 1:500-1:2000 ICC/IF: 1:200-1:500 FC: 1:20

Purification: Affinity Purification

Specificity: IgG

Molecular Weight: 50, 27 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q15465

Product: Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Research Use Only