SOX9 Rabbit Monoclonal Antibody-MB66294

SOX9 Rabbit Monoclonal Antibody

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Catalogue Numbers: MB66294-50, MB66294-100

Sizes: 50µl, 100µl

Swiss-Prot: P48436

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IP

Background: Sox9 is a transcription factor with an HMG-box DNA binding domain that has homology to the HMG domain of the mammalian testis-determining factor, SRY. Sox9 regulates several important processes during embryonic development including chondrogenesis, during which it contributes to skeletal formation and digit specification. Sox9 also coordinates with steroidogenic factor-1 to direct Sertoli cell-specific expression of anti-Mullerian hormone during embryogenesis, thereby contributing to male sex determination. In addition, Sox9 is reportedly involved in the maintenance of adult stem cell populations, including multipotent neural stem cells, hair follicle stem cells, and mammary stem cells. Recent interest has focused on the role of Sox9 in tumor biology. For example, research studies have shown that Sox9 expression in lung adenocarcinoma induces a mesenchymal phenotype in tumor cells. Other research studies have shown that YAP1 induced upregulation of Sox9 confers cancer stem cell like properties on esophageal cancer cells. Moreover, Sox9 expression has been linked with several other tumor types including ovarian, prostate, and pancreatic malignancies.

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of SOX9 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SOX9 expression in NIH3T3 (A) whole cell lysates., Immunohistochemical analysis of SOX9 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.6). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 70 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Transcription factor SOX-9

Immunogen: Recombinant protein of human SOX9

Conjugate: Unconjugated

Modification: Unmodified