Sp3/4 (G659) Polyclonal Antibody-BS2030

Sp3/4 (G659) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2030-50, BS2030-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02447/Q02446

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4(SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3,but not Sp2 or Sp4, interact with E2, a regulatory element for the β4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Sp3/4 (G659) polyclonal antibody detects endogenous levels of Sp3 and Sp4 protein.

Bioworld Molecular Weight: ~ 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Sp3/4 (G659) pAb at 1:1000 dilution
Lane 1: L02 whole cell lysate (20ug)
Lane 2: MCF-7 whole cell lysate (20ug)
Lane 3: H9C2 whole cell lysate (40ug)
Lane 4: MEF whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Sp3/4 (G659) pAb in paraffin-embedded human lung carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Sp3/4 (G659) pAb in paraffin-embedded human liver carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Sp3/4 (G659) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. Nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Transcription factor Sp3; SPR-2; SPR2; SP3; Transcription factor Sp4; SPR-1; SPR1; SP4

Immunogen: Synthetic peptide, corresponding to amino acids 630-680 of Human Sp3.

Conjugate: Unconjugated

Modification: Unmodified