Product Description

SPAG5 Polyclonal Antibody

Catalogue Numbers: BS60768-50, BS60768-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96R06

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST or Astrin, is a 1,193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SPAG5 polyclonal antibody detects endogenous levels of SPAG5 protein.

Extra Notes: Western blot (WB) analysis of SPAG5 pAb at 1:500 dilution
Lane1:Myla2059 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:HuT78 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 134, 150 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Sperm-associated antigen 5; Astrin; Deepest; Mitotic spindle-associated protein p126; MAP126; SPAG5

Immunogen: A synthetic peptide corresponding to residues in Human SPAG5

Conjugate: Unconjugated

Modification: Unmodified