Product Description

SPAG9 Polyclonal Antibody

Catalogue Numbers: BS60271-50, BS60271-100

Sizes: 50µl, 100µl

Alternative Name: C-Jun-amino-terminal kinase-interacting protein 4; JIP-4; JNK-interacting protein 4; Cancer/testis antigen 89; CT89; Human lung cancer oncogene 6 protein; HLC-6; JNK-associated leucine-zipper protein; JLP; Mitogen-activated protein kinase 8-interacting protein 4; Proliferation-inducing protein 6; Protein highly expressed in testis; PHET; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1; SPAG9; HSS, KIAA0516, MAPK8IP4, SYD1; HLC6

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60271

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: JIP-4 (C-jun-amino-terminal kinase-interacting protein 4, Mitogen-activated protein kinase 8-interacting protein 4, Sunday driver 1) is a 1,321 amino acid protein encoded by the human gene SPAG9. It contains a large N-terminal extracellular domain, a short transmembrane helical domain, and a cytoplasmic domain. There are 6 N-glycosylation sites, several phosphorylation sites for cAMP/cGMP-dependent protein kinase, protein kinase C, and casein kinase II, and 10 putative myristoylation sites. There is also a leucine zipper motif, with 6 leucine repeats, that may aid in dimerization since there is no upstream basic domain characteristic of DNA binding proteins. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP-4 is a cytoplasmic, perinuclear protein that has eight known isoforms whose expression varies by tissue and disease state.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SPAG9 polyclonal antibody detects endogenous levels of SPAG9 protein.

Bioworld Molecular Weight: ~ 146 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SPAG9 pAb at 1:500 dilution
Lane 1: AML-12 whole cell lysate(40ug)
Lane 2: U-87MG whole cell lysate(40ug)
Lane 3: EC9706 whole cell lysate(40ug)
Lane 4: SGC7901 whole cell lysate(40ug)

Immunogen: A synthetic peptide corresponding to residues in Human SPAG9.

Conjugate: Unconjugated

Modification: Unmodified