Product Description

SPAK Polyclonal Antibody

Catalogue Numbers: BS62488-50, BS62488-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UEW8

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitrostudies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible role for SPAK in the stress response. Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3. WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380. Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315. Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity. SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knock-down of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SPAK polyclonal antibody detects endogenous levels of SPAK protein.

Extra Notes: Western blot (WB) analysis of SPAK polyclonal antibody at 1:500 dilution
LaneA:CT26 whole cell lysate
LaneB:PC12 whole cell lysate
LaneC:A549 whole cell lysate
LaneD:MCF-7 whole cell lysate

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: STE20/SPS1-related proline-alanine-rich protein kinase; Ste-20-related kinase; DCHT; Serine/threonine-protein kinase 39; STK39; SPAK

Immunogen: Synthetic peptide, corresponding to Human SPAK.

Conjugate: Unconjugated

Modification: Unmodified