SPDYA Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75384-50, BS75384-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q5MJ70
Host: Rabbit
Reactivity: Human
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2. Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Mediates cell survival during the DNA damage process through activation of CDK2.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of A549 cells, using SPDYA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 50kDa
Alternative Name: SPDYA; RINGO3; RINGOA; SPDY1; SPY1
Immunogen: Recombinant fusion protein of human SPDYA(NP_877433.2).
Conjugate: Unconjugated
Modification: Unmodified