Product Description

SPHK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91277-50, BS91277-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NYA1(Human) Q8CI15(Mouse) Q91V26(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC FC

All Applications: WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50

Background: Sphingosine kinase (SphK or SphK1) is a key enzyme catalyzing the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP or S1P). Competitive inhibitors of SphK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. One potent inhibitor of SphK1 activity is DMS (N,N-dimethylsphingosine). SPP/SphK1 has been implicated as a signaling pathway that regulates diverse cellular functions, including cell growth, proliferation and survival. Specifically, SphK1 is involved in the signaling pathway(s) that protects human hepatocytes from the apoptotic action of TNFα. Furthermore, SPP/SphK1 may play an important role in neuronal survival by regulating activation of SAPKs and caspases. SphK1 is widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle; however, activation of SphK1 disengages cells from their liver-specific phenotype.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SPHK1 polyclonal antibody detects endogenous levels of SPHK1 protein.

Bioworld Molecular Weight: 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SPHK1 antibody. Counter stained with hematoxylin.

Alternative Name: SK 1, SPK 1, SPHK1, SPHK, SPK, SK 1, SK1, Sphingosine kinase 1, SPHK 1, SPHK, Sphk1, SPHK1, SPK, SPK 1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified