Product Description

Spindlin-1 (H139) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2678-50, BS2678-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9Y657

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: SPIN1 (spindlin 1), also known as SPIN or ovarian cancer-related protein (OCR), is a 262 amino acid nuclear protein suggested to play a role in regulation of the cell-cycle during the transition from gamete to embryo. A member of the SPIN/STSY family, SPIN1 localizes to interphase nucleus and mitotic chromosomes, and is modified by phosphorylation in a cell-cycle-dependent fashion. A meiotic spindle-binding protein, SPIN1 overexpression has been shown to cause defects in mitotic spindles, thereby resulting in chromosome instability and potential tumorigenesis.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Spindlin-1 (H139) polyclonal antibody detects endogenous levels of Spindlin-1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Spindlin-1 (H139) pAb at 1:500 dilution
Lane 1: SK-OVCAR3 whole cell lysate (40ug)
Lane 2: SGC7901 whole cell lysate (40ug)
Lane 3: The Ovarian tissue lysate of Rat (40ug)
Lane 4: The Testis tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of Spindlin-1 (H139) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 30 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: OCR; Ovarian cancer-related protein; SPIN; Spin1; SPIN1; Spindlin 1;

Immunogen: Synthetic peptide, corresponding to amino acids 110-156 of Human Spindlin-1.

Conjugate: Unconjugated

Modification: Unmodified